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Strain and process engineering toward continuous industrial fermentation

《化学科学与工程前沿（英文）》 2023年第17卷第10期页码 1336-1353 doi: 10.1007/s11705-022-2284-6

摘要： Most current biotechnology industries are based on batch or fed-batch fermentation processes, which often show low productivity and high production costs compared to chemical processes. To increase the economic competitiveness of biological processes, continuous fermentation technologies are being developed that offer significant advantages in comparison with batch/fed-batch fermentation processes, including: (1) removal of potential substrates and product inhibition, (2) prolonging the microbial exponential growth phase and enhancing productivity, and (3) avoiding repeated fermentation preparation and lowering operation and installation costs. However, several key challenges should be addressed for the industrial application of continuous fermentation processes, including (1) contamination of the fermentation system, (2) degeneration of strains, and (3) relatively low product titer. In this study, we reviewed and discussed metabolic engineering and synthetic biology strategies to address these issues.

关键词： continuous fermentation productivity contamination strain degeneration metabolic engineering

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Evolutionary engineering of

Jixian GONG, Nan DUAN, Xueming ZHAO

《化学科学与工程前沿（英文）》 2012年第6卷第2期页码 174-178 doi: 10.1007/s11705-012-1276-3

摘要： Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Astaxanthin has been widely used in cosmetics, pharmaceutical and health care food due to its capability of quenching active oxygen. Strain improvement of , one of the main sources for natural astaxanthin, is of commercial interest for astaxanthin production. In this study a selection procedure was developed for adaptive evolution of strains under endogenetic selective pressure induced by additive in environmental niches. Six agents, which can induce active oxygen in cells, were added to the culture medium respectively to produce selective pressure in process of evolution. The initial strain, 2-1557, was mutagenized to acquire the initial strain population, which was then cultivated for 550 h at selective pressure and the culture was transferred every 48h. Finally, six evolved strains were selected after 150 generations of evolution. The evolved strains produced up to 48.2% more astaxanthin than the initial strain. Our procedure may provide a promising alternative for improvement of high-production strain.

关键词： evolutionary engineering astaxanthin strain improvement

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Concepts and implementation of strain-based criteria in design codes for steel structures

Reidar BJORHOVDE

《结构与土木工程前沿（英文）》 2012年第6卷第3期页码 210-216 doi: 10.1007/s11709-012-0165-7

摘要： A uniaxial tension test is commonly used to determine the mechanical properties of steel, but it has no meaning for the response of the material in a structure. The test was developed as a consensus solution by producers, fabricators, designers and code writers, to have a standard by which similar materials could be compared to a common base. It does not represent the actual behavior of the steel in a structure, and was never intended to do so. To study the true behavior of the structure and how the material responds it would be better to determine the strains and deformations that will take place during actual service condition. Such characteristics reflect the real behavior, whether in the elastic or inelastic range. If stresses or forces are needed, these are easily determined by the value of the strain and the relevant material modulus, along with the type of cross section, whether elastic or inelastic. The paper addresses the properties of a range of structural steels, how these are incorporated into design standards and how the standards define deformation characteristics and demands for bolted and welded connections.

关键词： steel stress-strain characteristics tension test strain design actual behavior improved design codes

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工程爆破节能环保技术研究与应用

刘友平,李义,张丕界,何广沂

《中国工程科学》 2008年第10卷第9期页码 39-46

摘要：

工程爆破节能环保技术的核心内容是往炮眼中一定位置注入一定量的水并用专用设备制成的炮泥回填堵塞，与常规工程爆破相比，具有显著的节能环保作用。主要介绍了往炮眼底部注水在实际应用中所产生的作用与效果并对此进行了分析，而且通过模拟实验应变测试结果证明了理论分析的正确性。

关键词：工程水压爆破节能环保模拟实验应变测试

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Full-field dynamic strain reconstruction of an aero-engine blade from limited displacement responses

《机械工程前沿（英文）》 2023年第18卷第1期 doi: 10.1007/s11465-022-0731-1

摘要： Blade strain distribution and its change with time are crucial for reliability analysis and residual life evaluation in blade vibration tests. Traditional strain measurements are achieved by strain gauges (SGs) in a contact manner at discrete positions on the blades. This study proposes a method of full-field and real-time strain reconstruction of an aero-engine blade based on limited displacement responses. Limited optical measured displacement responses are utilized to reconstruct the full-field strain. The full-field strain distribution is in-time visualized. A displacement-to-strain transformation matrix is derived on the basis of the blade mode shapes in the modal coordinate. The proposed method is validated on an aero-engine blade in numerical and experimental cases. Three discrete vibrational displacement responses measured by laser triangulation sensors are used to reconstruct the full-field strain over the whole operating time. The reconstructed strain responses are compared with the results measured by SGs and numerical simulation. The high consistency between the reconstructed and measured results demonstrates the accurate strain reconstructed by the method. This paper provides a low-cost, real-time, and visualized measurement of blade full-field dynamic strain using displacement response, where the traditional SGs would fail.

关键词： aero-engine blade displacement response dynamic strain reconstruction mode shape strain gauge

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机械与材料力学性能的三维全场变形与应变快速检测研究

梁晋,郭翔,胡浩,李磊刚,唐正宗,史宝全,孙涛

《中国工程科学》 2013年第15卷第1期页码 51-56

摘要：

提出并实现了一种基于工业摄影测量和数字图像的相关法，通过多种工业相机拍摄的多幅二维序列图像，快速解算出被测物体的三维坐标、变形和应变数据的方法。该方法解决了大型复杂工件生产现场快速检测难题，达到国际先进水平，可用于中小型工件和微纳米尺度工件的相关检测，以及材料性能分析，也可满足低速到高速的振动冲击和模态分析要求。在机械、材料力学等多个行业和多个学科的应用表明，该方法具有适用面广、三维全场检测、快速灵活方便的优点，取得了显著的经济和社会效益。

关键词：工业摄影测量数字图像相关法变形应变

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Behavior of steel fiber–reinforced high-strength concrete at medium strain rate

Chujie JIAO, Wei SUN, Shi HUAN, Guoping JIANG

《结构与土木工程前沿（英文）》 2009年第3卷第2期页码 131-136 doi: 10.1007/s11709-009-0027-0

摘要： Impact compression experiments for the steel fiber–reinforced high-strength concrete (SFRHSC) at medium strain rate were conducted using the split Hopkinson press bar (SHPB) testing method. The volume fractions of steel fibers of SFRHSC were between 0 and 3%. The experimental results showed that, when the strain rate increased from threshold value to 90 s , the maximum stress of SFRHSC increased about 30%, the elastic modulus of SFRHSC increased about 50%, and the increase in the peak strain of SFRHSC was 2-3 times of that in the matrix specimen. The strength and toughness of the matrix were improved remarkably because of the superposition effect of the aggregate high-strength matrix and steel fiber high-strength matrix. As a result, under impact loading, cracks developed in the SFRHSC specimen, but the overall shape of the specimen remained virtually unchanged. However, under similar impact loading, the matrix specimens were almost broken into small pieces.

关键词： steel fiber–reinforced high-strength concrete (SFRHSC) high strain rates split Hopkinson press bar (SHPB) strain rate hardening effects

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Vibration analysis of nano-structure multilayered graphene sheets using modified strain gradient theory

Amir ALLAHBAKHSHI,Masih ALLAHBAKHSHI

《机械工程前沿（英文）》 2015年第10卷第2期页码 187-197 doi: 10.1007/s11465-015-0339-9

摘要：

In this paper, for the first time, the modified strain gradient theory is used as a new size-dependent Kirchhoff micro-plate model to study the effect of interlayer van der Waals (vdW) force for the vibration analysis of multilayered graphene sheets (MLGSs). The model contains three material length scale parameters, which may effectively capture the size effect. The model can also degenerate into the modified couple stress plate model or the classical plate model, if two or all of the material length scale parameters are taken to be zero. After obtaining the governing equations based on modified strain gradient theory via principle of minimum potential energy, as only infinitesimal vibration is considered, the net pressure due to the vdW interaction is assumed to be linearly proportional to the deflection between two layers. To solve the governing equation subjected to the boundary conditions, the Fourier series is assumed for w=w(x, y). To show the accuracy of the formulations, present results in specific cases are compared with available results in literature and a good agreement can be seen. The results indicate that the present model can predict prominent natural frequency with the reduction of structural size, especially when the plate thickness is on the same order of the material length scale parameter.

关键词： graphene van der Waals (vdW) force modi- fied strain gradient elasticity theory size effect parameter

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FEM-based strain analysis study for multilayer sheet forming process

Rongjing ZHANG,Lihui LANG,Rizwan ZAFAR

《机械工程前沿（英文）》 2015年第10卷第4期页码 373-379 doi: 10.1007/s11465-015-0371-9

摘要：

Fiber metal laminates have many advantages over traditional laminates (e.g., any type of fiber and resin material can be placed anywhere between the metallic layers without risk of failure of the composite fabric sheets). Furthermore, the process requirements to strictly control the temperature and punch force in fiber metal laminates are also less stringent than those in traditional laminates. To further explore the novel method, this study conducts a finite element method-based (FEM-based) strain analysis on multilayer blanks by using the 3A method. Different forming modes such as wrinkling and fracture are discussed by using experimental and numerical studies. Hydroforming is used for multilayer forming. The Barlat 2000 yield criteria and DYNAFORM/LS-DYNA are used for the simulations. Optimal process parameters are determined on the basis of fixed die-binder gap and variable cavity pressure. The results of this study will enhance the knowledge on the mechanics of multilayer structures formed by using the 3A method and expand its commercial applications.

关键词： finite element method (FEM) strain analysis multilayer sheet forming

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Stress-strain relationship of concrete in freeze-thaw environment

ZOU Chaoying, ZHAO Juan, LIANG Feng

《结构与土木工程前沿（英文）》 2008年第2卷第2期页码 184-188 doi: 10.1007/s11709-008-0029-3

摘要： Adopting the ASTM C666 quick freeze-thaw method, freeze-thaw tests with the number of freeze-thaw cycles being 0, 100, 150, 200, 250, and 300, were carried out on eighteen concrete prism specimens, the frost-resistant level of which, reaches D300 grade. The internal micro-structures of these specimens were observed by means of scanning electron microscopy (SEM) in order to detect damages caused by the freeze-thaw action. Afterwards these frozen-thawed specimens were tested on monotonic axial load; stress-strain testing curves after the freeze-thaw action were obtained. The testing results show that with the increasing time of freeze-thaw cycles, both the internal micro-structures and the basic mechanical parameters, including the ultimate bearing capacity, the Poisson ratio and the modulus of elasticity, degenerate in various degrees. Finally, stress-strain theoretical curvilinear equations and correlated parameters relating to the loss of relative dynamic modulus of elasticity were proposed. These results provide great reference for further research on the behavior and the calculation model of concrete structures in a freeze-thaw environment.

关键词： stress-strain theoretical freeze-thaw capacity reference eighteen concrete

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Fictitious soil pile model for dynamic analysis of pipe piles under high-strain conditions

《结构与土木工程前沿（英文）》页码 915-934 doi: 10.1007/s11709-023-0907-8

摘要： A fictitious soil pile (FSP) model is developed to simulate the behavior of pipe piles with soil plugs undergoing high-strain dynamic impact loading. The developed model simulates the base soil with a fictitious hollow pile fully filled with a soil plug extending at a cone angle from the pile toe to the bedrock. The friction on the outside and inside of the pile walls is distinguished using different shaft models, and the propagation of stress waves in the base soil and soil plug is considered. The motions of the pile−soil system are solved by discretizing them into spring-mass model based on the finite difference method. Comparisons of the predictions of the proposed model and conventional numerical models, as well as measurements for pipe piles in field tests subjected to impact loading, validate the accuracy of the proposed model. A parametric analysis is conducted to illustrate the influence of the model parameters on the pile dynamic response. Finally, the effective length of the FSP is proposed to approximate the affected soil zone below the pipe pile toe, and some guidance is provided for the selection of the model parameters.

关键词： fictitious soil pile soil plug pipe piles high-strain dynamic analysis one-dimensional wave theory pile dynamics

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Influence of freeze–thaw damage gradient on stress–strain relationship of stressed concrete

《结构与土木工程前沿（英文）》页码 1326-1340 doi: 10.1007/s11709-023-0014-x

摘要： Influence of freeze–thaw damage gradient on stress–strain relationship of stressed concrete

关键词： strain relationship concrete

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Separation and extraction of bridge dynamic strain data

Baijian WU, Zhaoxia LI, Ying WANG, T. H. T. CHAN,

《结构与土木工程前沿（英文）》 2009年第3卷第4期页码 395-400 doi: 10.1007/s11709-009-0049-7

摘要： Through comparing the measured data of dynamic strains due to loading and temperature by the stain gauge and temperature sensor at the same location, the information in the strain data was divided into three parts in the frequency domain by using the defined index named power spectral density (PSD)-ratio index. The three parts are dominated respectively by temperature varying, stresses, and noises and thus can be distinguished from the determined the separatrix frequencies. Also, a simple algorithm was developed to separate the three types of information and to extract the strain caused mainly by structural stresses. As an application of the proposed method, the effect of strain deformation and noises on the fatigue assessment was investigated based on the separated data. The results show that, the determined values of separatrix frequencies are valuable for the monitoring data from other bridges. The algorithm is a multiresolution and hierarchical method, which has been validated as a simple and effective method for data analyses, and is suitable for the compression and preprocessing of the great amount monitoring data and easy to be integrated into the structural health monitoring (SHM) soft system. The strain due to temperature varying attributes a little to the errors of fatigue assessment; however, the noises or random disturbance existed in the monitoring data have much responsibility for the errors, and the main reason is that the random disturbance shifts the real strain/stress amplitude picked up by real structural stress or strain.

关键词： dynamic valuable separatrix strain/stress amplitude frequency

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Strengthening of the concrete face slabs of dams using sprayable strain-hardening fiber-reinforced cementitious

《结构与土木工程前沿（英文）》 2022年第16卷第2期页码 145-160 doi: 10.1007/s11709-022-0806-4

摘要： In this study, sprayable strain-hardening fiber-reinforced cementitious composites (FRCC) were applied to strengthen the concrete slabs in a concrete-face rockfill dam (CFRD) for the first time. Experimental, numerical, and analytical investigations were carried out to understand the flexural properties of FRCC-layered concrete slabs. It was found that the FRCC layer improved the flexural performance of concrete slabs significantly. The cracking and ultimate loads of a concrete slab with an 80 mm FRCC layer were 132% and 69% higher than those of the unstrengthened concrete slab, respectively. At the maximum crack width of 0.2 mm, the deflection of the 80-mm FRCC strengthened concrete slab was 144% higher than that of the unstrengthened concrete slab. In addition, a FE model and a simplified analytical method were developed for the design and analysis of FRCC-layered concrete slabs. Finally, the test result of FRCC leaching solution indicated that the quality of the water surrounding FRCC satisfied the standard for drinking water. The findings of this study indicate that the sprayable strain-hardening FRCC has a good potential for strengthening hydraulic structures such as CFRDs.

关键词： strain-hardening cementitious composites engineered cementitious composites sprayable shotcrete strengthening concrete-face rockfill dam digital image correlation

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Aerobic granulation of pure bacterial strain

ADAV Sunil S., LEE Duu-Jong

《环境科学与工程前沿（英文）》 2008年第2卷第4期页码 461-467 doi: 10.1007/s11783-008-0066-0

摘要： The objective of this study is to cultivate aerobic granules by pure bacterial strain, , in a sequencing batch reactor. Stable granules sized 2.0–2.2 mm were formed in the reactor after a five-week cultivation. These granules exhibited excellent settling attributes, and degraded phenol at rates of 1.49 and 1.19 g phenol/(g VSS·d) at 250 and 1500 mg/L of phenol concentration, respectively. Confocal laser scanning microscopic test results show that was distributed over the initial small aggregates, and the outer edge of the granule was away from the core regime in the following stage.

关键词： following excellent settling phenol/ sequencing microscopic